The 8-epi-PGF2 alpha (8-epi-Prostaglandin F2 alpha) ELISA Kit is specially designed for the precise measurement of 8-epi-PGF2 alpha levels in various biological samples including serum, plasma, and urine. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers working in the fields of oxidative stress, inflammation, and lipid peroxidation.8-epi-PGF2 alpha is a biomarker of oxidative stress and lipid peroxidation, playing a crucial role in the pathogenesis of various diseases such as cardiovascular disorders, asthma, and chronic kidney disease.
By accurately quantifying 8-epi-PGF2 alpha levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the 8-epi-PGF2 alpha ELISA Kit is an essential tool for studying oxidative stress-related diseases and developing novel treatment strategies to improve patient outcomes.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-epi-PGF2α. During the reaction, 8-epi-PGF2α in the sample or standard competes with a fixed amount of 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-epi-PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
87-102
88-99
Average (%)
91
94
94
1:4
Range (%)
83-95
87-100
94-111
Average (%)
90
93
101
1:8
Range (%)
85-101
88-101
98-114
Average (%)
92
95
105
1:16
Range (%)
83-94
85-99
99-114
Average (%)
89
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
92
EDTA plasma (n=5)
92-108
100
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.2
101.8
446.4
54.5
95.7
463.7
Standard deviation
2.9
4.5
15.6
3.5
5.0
25.0
C V (%)
5.66
4.42
3.49
6.42
5.22
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes 8-epi-PGF2α in samples. No significant cross-reactivity or interference between 8-epi-PGF2α and analogues was observed.
