The 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit is specifically designed for the accurate and precise detection of 5-HIAA levels in various biological samples including urine, plasma, and cerebrospinal fluid. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.5-HIAA is a metabolite of serotonin and is commonly used as a biomarker for various neurological and psychiatric disorders, including carcinoid tumors and serotonin syndrome.
Monitoring 5-HIAA levels can provide valuable insights into the serotonin pathway and help in the diagnosis and treatment of these conditions.With its easy-to-use protocol and excellent performance characteristics, the 5-HIAA ELISA Kit is a valuable tool for researchers and healthcare professionals studying serotonin metabolism and related disorders.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 5-HIAA. During the reaction, 5-HIAA in the sample or standard competes with a fixed amount of 5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-HIAA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 5-HIAA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
94-107
88-98
Average (%)
100
101
93
1:4
Range (%)
95-106
88-100
89-104
Average (%)
101
94
95
1:8
Range (%)
94-110
95-106
85-97
Average (%)
102
100
91
1:16
Range (%)
96-109
93-108
88-100
Average (%)
102
100
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
93-106
98
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.2
588.5
1720.2
221.1
588.2
1852.0
Standard deviation
10.6
29.4
61.9
13.9
24.7
59.3
C V (%)
5.19
5.0
3.6
6.29
4.2
3.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of 5-HIAA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes 5-HIAA in samples. No significant cross-reactivity or interference between 5-HIAA and analogues was observed.
