TEV Recombinant Protein (RPPB5123)
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주문- SKU:
- RPPB5123
- Product type:
- Recombinant Protein
- Size:
- 500IU
- Target:
- TEV
- Synonyms:
- rTEV
- Synonyms:
- TEV
- Synonyms:
- P1 protease
- Source:
- Escherichia Coli
- Uniprot:
- Q0GDU8
Description
Product Name: | TEV Recombinant Protein |
Product Code: | RPPB5123 |
Size: | 500IU |
Target: | TEV |
Synonyms: | rTEV, TEV, P1 protease. |
Source: | Escherichia Coli |
Physical Appearance: | Sterile liquid formulation. |
Formulation: | The rTEV contains 25mM Tris, 75mM NaCl, 5mM EDTA, 10mM GSH, 50% Glycerol. |
Stability: | rTEV although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
Purity: | Greater than 90.0% as determined by analysis by SDS-PAGE. |
UniProt Code: | Q0GDU8 |
TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.
Recombinant TEV Protease (rTEV) is a site-specific protease purified from E. coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30°C; however, the enzyme can be used at temperatures as low as 4°C. The rTEV contains His tag.The rTEV is purified by proprietary chromatographic techniques.