SYNJ1 Antibody (PACO57996)
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주문- SKU:
- PACO57996
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | SYNJ1 Antibody (PACO57996) |
Antibody SKU: | PACO57996 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Synaptojanin-1 protein (1030-1261AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO57996 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Western Blot. Positive WB detected in: HT29 whole cell lysate. All lanes: SYNJ1 antibody at 3.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 174, 145, 144, 59 kDa. Observed band size: 174 kDa. |
![]() | Immunofluorescence staining of NIH/3T3 cells with PACO57996 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Phosphatase that acts on various phosphoinositides, including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate. Has a role in clathrin-mediated endocytosis (By similarity). Hydrolyzes PIP2 bound to actin regulatory proteins resulting in the rearrangement of actin filaments downstream of tyrosine kinase and ASH/GRB2 (By similarity). |
Synonyms: | Synaptojanin-1 (EC 3.1.3.36) (Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1), SYNJ1, KIAA0910 |
UniProt Protein Function: | SYNJ1: Inositol 5-phosphatase which has a role in clathrin- mediated endocytosis. Belongs to the synaptojanin family. 4 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Phosphatase, lipid; EC 3.1.3.36; Carbohydrate Metabolism - inositol phosphate; Vesicle; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 21q22.2 Cellular Component: clathrin coat of coated pit; cytosol; membrane coat; microtubule; terminal button; vesicle membrane Molecular Function:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity; phosphatidylinositol-3-phosphatase activity; phosphatidylinositol-4-phosphate phosphatase activity; phosphoinositide 5-phosphatase activity Biological Process: inositol phosphate dephosphorylation; inositol phosphate metabolic process; learning; neurotransmitter transport; phosphatidylinositol biosynthetic process; phosphatidylinositol metabolic process; phosphoinositide dephosphorylation; synaptic vesicle endocytosis; synaptic vesicle priming; synaptic vesicle transport; synaptic vesicle uncoating Disease: Parkinson Disease 20, Early-onset |
NCBI Summary: | This gene encodes a phosphoinositide phosphatase that regulates levels of membrane phosphatidylinositol-4,5-bisphosphate. As such, expression of this enzyme may affect synaptic transmission and membrane trafficking. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
UniProt Code: | O43426 |
NCBI GenInfo Identifier: | 215273894 |
NCBI Gene ID: | 8867 |
NCBI Accession: | O43426.2 |
UniProt Secondary Accession: | O43426,O43425, O94984, Q4KMR1, |
UniProt Related Accession: | O43426 |
Molecular Weight: | 58,068 Da |
NCBI Full Name: | Synaptojanin-1 |
NCBI Synonym Full Names: | synaptojanin 1 |
NCBI Official Symbol: | SYNJ1 |
NCBI Official Synonym Symbols: | INPP5G; PARK20 |
NCBI Protein Information: | synaptojanin-1 |
UniProt Protein Name: | Synaptojanin-1 |
UniProt Synonym Protein Names: | Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1 |
Protein Family: | Synaptojanin |
UniProt Gene Name: | SYNJ1 |
UniProt Entry Name: | SYNJ1_HUMAN |
Antibodies |
Anti-SYNJ1 Antibody (CAB17581) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |