SRPK2 Antibody (PACO62987)
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주문- SKU:
- PACO62987
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | SRPK2 Antibody (PACO62987) |
Antibody SKU: | PACO62987 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human SRSF protein kinase 2 protein (471-688AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO62987 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate. All lanes: SRPK2 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 78, 80 kDa. Observed band size: 78 kDa. |
Background: | Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. |
Synonyms: | SRSF protein kinase 2 (EC 2.7.11.1) (SFRS protein kinase 2) (Serine/arginine-rich protein-specific kinase 2) (SR-protein-specific kinase 2) [Cleaved into: SRSF protein kinase 2 N-terminal; SRSF protein kinase 2 C-terminal], SRPK2 |
UniProt Protein Function: | SRPK2: a CMGC kinase of the SRPK family. Phosphorylates the SR (serine/arginine-rich domain) family of splicing factors. Localizes to the nucleus and the cytoplasm. Thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. Highly expressed in brain and to a lesser extent other tissues. A second alternatively spliced transcript variant has been described, but its full length nature has not been determined. |
UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, CMGC; Kinase, protein; CMGC group; SRPK family Chromosomal Location of Human Ortholog: 7q22-q31.1 Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus Molecular Function:ATP binding; magnesium ion binding; protein binding; protein serine/threonine kinase activity Biological Process: angiogenesis; innate immune response; negative regulation of viral genome replication; nuclear speck organization and biogenesis; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of neuron apoptosis; positive regulation of viral genome replication; protein amino acid phosphorylation; regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; spliceosome assembly |
UniProt Code: | P78362 |
NCBI GenInfo Identifier: | 300669676 |
NCBI Gene ID: | 6733 |
NCBI Accession: | P78362.3 |
UniProt Secondary Accession: | P78362,O75220, O75221, Q6NUL0, Q6V1X2, Q8IYQ3, A8MVX2 |
UniProt Related Accession: | P78362 |
Molecular Weight: | 79,029 Da |
NCBI Full Name: | SRSF protein kinase 2 |
NCBI Synonym Full Names: | SRSF protein kinase 2 |
NCBI Official Symbol: | SRPK2 |
NCBI Official Synonym Symbols: | SFRSK2 |
NCBI Protein Information: | SRSF protein kinase 2 |
UniProt Protein Name: | SRSF protein kinase 2 |
UniProt Synonym Protein Names: | SFRS protein kinase 2; Serine/arginine-rich protein-specific kinase 2; SR-protein-specific kinase 2 |
Protein Family: | SRSF protein kinase |
UniProt Gene Name: | SRPK2 |
UniProt Entry Name: | SRPK2_HUMAN |
Antibodies |
Anti-SRPK2 Antibody (CAB17536) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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