SNX5 Antibody (PACO63219)
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주문- SKU:
- PACO63219
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | SNX5 Antibody (PACO63219) |
Antibody SKU: | PACO63219 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Sorting nexin-5 protein (7-245AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO63219 diluted at 1:640 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate. All lanes: SNX5 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 14 kDa. Observed band size: 47 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO63219 at 1:213, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Does not have in vitro vesicle-to-membrane remodeling activity. Involved in retrograde transport of lysosomal enzyme receptor IGF2R. May function as link between endosomal transport vesicles and dynactin (Probable). Plays a role in the internalization of EGFR after EGF stimulation (Probable). Involved in EGFR endosomal sorting and degradation; the function involves PIP5K1C isoform 3 and is retromer-independent. Together with PIP5K1C isoform 3 facilitates HGS interaction with ubiquitinated EGFR, which initiates EGFR sorting to intraluminal vesicles (ILVs) of the multivesicular body for subsequent lysosomal degradation (Probable). Involved in E-cadherin sorting and degradation; inhibits PIP5K1C isoform 3-mediated E-cadherin degradation. Plays a role in macropinocytosis. |
Synonyms: | Sorting nexin-5, SNX5 |
UniProt Protein Function: | SNX5: May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation. Belongs to the sorting nexin family. |
UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 20p11 Cellular Component: ruffle; extrinsic to internal side of plasma membrane; cytoplasmic vesicle membrane; early endosome membrane; retromer complex; phagocytic cup Molecular Function:protein binding; phosphoinositide binding Biological Process: pinocytosis; intracellular protein transport; vesicle organization and biogenesis; retrograde transport, endosome to Golgi |
NCBI Summary: | This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein functions in endosomal sorting, the phosphoinositide-signaling pathway, and macropinocytosis. This gene may play a role in the tumorigenesis of papillary thyroid carcinoma. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013] |
UniProt Code: | Q9Y5X3 |
NCBI GenInfo Identifier: | 10720289 |
NCBI Gene ID: | 27131 |
NCBI Accession: | Q9Y5X3.1 |
UniProt Secondary Accession: | Q9Y5X3,Q52LC4, Q7KZN0, Q9BWP0, B7ZKN3, D3DW26, |
UniProt Related Accession: | Q9Y5X3 |
Molecular Weight: | 404 |
NCBI Full Name: | Sorting nexin-5 |
NCBI Synonym Full Names: | sorting nexin 5 |
NCBI Official Symbol: | SNX5 |
NCBI Protein Information: | sorting nexin-5 |
UniProt Protein Name: | Sorting nexin-5 |
Protein Family: | Sorting nexin |
UniProt Gene Name: | SNX5 |
UniProt Entry Name: | SNX5_HUMAN |