RHOU Antibody (PACO57228)
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주문- SKU:
- PACO57228
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | RHOU Antibody (PACO57228) |
Antibody SKU: | PACO57228 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Rho-related GTP-binding protein RhoU protein (1-48AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO57228 diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of HepG2 cells with PACO57228 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO57228 diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. |
Synonyms: | Rho-related GTP-binding protein RhoU (CDC42-like GTPase 1) (GTP-binding protein-like 1) (Rho GTPase-like protein ARHU) (Ryu GTPase) (Wnt-1 responsive Cdc42 homolog 1) (WRCH-1), RHOU, ARHU CDC42L1 G28K WRCH1 |
UniProt Protein Function: | Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. |
NCBI Summary: | This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011] |
UniProt Code: | Q7L0Q8 |
NCBI GenInfo Identifier: | 11034843 |
NCBI Gene ID: | 58480 |
NCBI Accession: | NP_067028.1 |
UniProt Secondary Accession: | Q7L0Q8,Q59FE9, Q8TDQ2, B1AKN1, |
UniProt Related Accession: | Q7L0Q8 |
Molecular Weight: | 22,164 Da |
NCBI Full Name: | rho-related GTP-binding protein RhoU |
NCBI Synonym Full Names: | ras homolog family member U |
NCBI Official Symbol: | RHOU |
NCBI Official Synonym Symbols: | ARHU; G28K; WRCH1; hG28K; CDC42L1 |
NCBI Protein Information: | rho-related GTP-binding protein RhoU |
UniProt Protein Name: | Rho-related GTP-binding protein RhoU |
UniProt Synonym Protein Names: | CDC42-like GTPase 1; GTP-binding protein-like 1; Rho GTPase-like protein ARHU; Ryu GTPase; Wnt-1 responsive Cdc42 homolog 1; WRCH-1 |
Protein Family: | Rho-related GTP-binding protein |
UniProt Gene Name: | RHOU |