RECQL4 Antibody (PACO58861)
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주문- SKU:
- PACO58861
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | RECQL4 Antibody (PACO58861) |
Antibody SKU: | PACO58861 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human ATP-dependent DNA helicase Q4 protein (200-337AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate. All lanes: RECQL4 antibody at 4.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 134 kDa. Observed band size: 134 kDa. |
![]() | Immunofluorescence staining of HepG2 cells with PACO58861 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO58861 diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | DNA-dependent ATPase. May modulate chromosome segregation. |
Synonyms: | ATP-dependent DNA helicase Q4 (EC 3.6.4.12) (DNA helicase, RecQ-like type 4) (RecQ4) (RTS) (RecQ protein-like 4), RECQL4, RECQ4 |
UniProt Protein Function: | RECQL4: DNA-dependent ATPase. May modulate chromosome segregation. Interacts with UBR1 and UBR2. Interacts with MCM10; this interaction regulates RECQL4 unwinding activity. Up-regulated in actively dividing cells. Ubiquitously expressed, with highest levels in thymus and testis. Belongs to the helicase family. RecQ subfamily. |
UniProt Protein Details: | Protein type:Helicase; EC 3.6.4.12 Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: membrane; cytoplasm; nucleus Molecular Function:ATP-dependent 3'-5' DNA helicase activity; zinc ion binding; bubble DNA binding; ATP binding Biological Process: multicellular organismal development; DNA strand renaturation; DNA replication; DNA repair; DNA duplex unwinding; DNA recombination Disease: Rapadilino Syndrome; Rothmund-thomson Syndrome; Baller-gerold Syndrome |
NCBI Summary: | The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq, Jan 2010] |
UniProt Code: | O94761 |
NCBI GenInfo Identifier: | 18206225 |
NCBI Gene ID: | 9401 |
NCBI Accession: | O94761.1 |
UniProt Secondary Accession: | O94761,Q3Y424, Q96DW2, Q96F55, |
UniProt Related Accession: | O94761 |
Molecular Weight: | 1208 |
NCBI Full Name: | ATP-dependent DNA helicase Q4 |
NCBI Synonym Full Names: | RecQ protein-like 4 |
NCBI Official Symbol: | RECQL4 |
NCBI Official Synonym Symbols: | RECQ4 |
NCBI Protein Information: | ATP-dependent DNA helicase Q4; DNA helicase, RecQ-like type 4; DNA helicase, RecQ-like, type 4 |
UniProt Protein Name: | ATP-dependent DNA helicase Q4 |
UniProt Synonym Protein Names: | DNA helicase, RecQ-like type 4; RecQ4; RTS; RecQ protein-like 4 |
Protein Family: | ATP-dependent DNA helicase |
UniProt Gene Name: | RECQL4 |
UniProt Entry Name: | RECQ4_HUMAN |