RBL2 Antibody (PACO62443)
- SKU:
- PACO62443
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | RBL2 Antibody (PACO62443) |
Antibody SKU: | PACO62443 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Retinoblastoma-like protein 2 protein (417-616AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO62443 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. |
Synonyms: | Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (p130) (Retinoblastoma-related protein 2) (RBR-2) (pRb2), RBL2, RB2 |
UniProt Protein Function: | Rb-like 2: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. Belongs to the retinoblastoma protein (RB) family. |
UniProt Protein Details: | Protein type:Tumor suppressor; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 16q12.2 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nucleus Molecular Function:protein binding; DNA binding Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of cell cycle; regulation of lipid kinase activity; chromatin modification; mitotic cell cycle |
UniProt Code: | Q08999 |
NCBI GenInfo Identifier: | 116242746 |
NCBI Gene ID: | 5934 |
NCBI Accession: | Q08999.3 |
UniProt Secondary Accession: | Q08999,Q15073, Q16084, Q8NE70, Q92812, B7Z913, |
UniProt Related Accession: | Q08999 |
Molecular Weight: | 59,349 Da |
NCBI Full Name: | Retinoblastoma-like protein 2 |
NCBI Synonym Full Names: | retinoblastoma-like 2 |
NCBI Official Symbol: | RBL2 |
NCBI Official Synonym Symbols: | Rb2; P130 |
NCBI Protein Information: | retinoblastoma-like protein 2; PRB2; RBR-2; retinoblastoma-related protein 2; 130 kDa retinoblastoma-associated protein |
UniProt Protein Name: | Retinoblastoma-like protein 2 |
UniProt Synonym Protein Names: | 130 kDa retinoblastoma-associated protein; p130; Retinoblastoma-related protein 2; RBR-2; pRb2 |
Protein Family: | RHOMBOID-like protein |
UniProt Gene Name: | RBL2 |
UniProt Entry Name: | RBL2_HUMAN |