Rat TGF- beta1 (Transforming Growth Factor beta1) CLIA Kit
The Rat TGF-beta1 (Transforming Growth Factor beta1) CLIA Kit is a highly sensitive assay designed for the quantitative measurement of TGF-beta1 levels in rat serum, plasma, and cell culture supernatants. This kit offers superior specificity and accuracy, providing researchers with reliable and reproducible results for a variety of experimental applications. TGF-beta1 is a key regulatory cytokine that plays a crucial role in cell growth, differentiation, and immune response. Dysregulation of TGF-beta1 signaling has been implicated in various diseases, including cancer, fibrosis, and autoimmune disorders.
This kit allows for the precise quantification of TGF-beta1 levels, enabling researchers to study its role in disease pathogenesis and evaluate potential therapeutic interventions. With its user-friendly protocol and robust performance, the Rat TGF-beta1 CLIA Kit is an indispensable tool for investigating the biological functions of TGF-beta1 in rat models and advancing our understanding of its impact on health and disease. Order yours today to accelerate your research efforts and uncover new insights into this critical signaling pathway.
Product Name:
Rat TGF- beta1 (Transforming Growth Factor beta1) CLIA Kit
SKU:
RTES00052
Target:
Rat TGF- beta1 (Transforming Growth Factor beta1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-β1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TGF-β1. You can calculate the concentration of Rat TGF-β1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
99-114
91-105
Average (%)
109
106
97
1:4
Range (%)
91-105
92-105
85-97
Average (%)
99
98
92
1:8
Range (%)
92-108
94-106
102-115
Average (%)
98
101
108
1:16
Range (%)
86-98
101-116
86-98
Average (%)
91
108
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
94-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.15
87.21
352.61
41.27
88.49
353.17
Standard deviation
3.94
7.86
40.23
5.11
10.13
39.94
C V (%)
9.81
9.01
11.41
12.38
11.45
11.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TGF-β1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TGF-β1 in samples. No significant cross-reactivity or interference between Rat TGF-β1 and analogues was observed.
