The Rat SHBG (Sex Hormone Binding Globulin) ELISA Kit is specifically designed for the quantitative measurement of SHBG levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.SHBG is a critical protein that binds to sex hormones such as testosterone and estrogen, regulating their bioavailability and activity. Changes in SHBG levels have been associated with various hormonal disorders, metabolic diseases, and reproductive health conditions.
By using this ELISA kit, researchers can study the role of SHBG in these conditions and explore potential therapeutic interventions.Overall, the Rat SHBG ELISA Kit provides a valuable tool for investigating the role of SHBG in physiological processes and pathological conditions, offering researchers a deeper understanding of hormonal regulation and potential treatment strategies.
Product Name:
Rat SHBG (Sex Hormone-Binding Globulin) ELISA Kit
SKU:
RTES00736
Target:
Rat SHBG (Sex Hormone-Binding Globulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SHBG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SHBG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SHBG. You can calculate the concentration of Rat SHBG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
88-102
96-113
Average (%)
96
95
104
1:4
Range (%)
93-105
83-95
87-102
Average (%)
98
88
94
1:8
Range (%)
93-106
85-100
83-96
Average (%)
100
92
87
1:16
Range (%)
91-107
80-90
84-97
Average (%)
98
85
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.63
141.9
414.51
47.89
130.99
431.49
Standard deviation
2.6
6.9
15.3
3.14
6.48
22.78
C V (%)
5.04
4.86
3.69
6.56
4.95
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SHBG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SHBG in samples. No significant cross-reactivity or interference between Rat SHBG and analogues was observed.
