The Rat PCT (Procalcitonin) ELISA Kit is specifically designed for the precise measurement of procalcitonin levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it perfect for various research applications.Procalcitonin is an important biomarker linked to systemic inflammation and bacterial infections. Its levels rise significantly in response to bacterial infections, making it a valuable tool for diagnosing sepsis or monitoring antibiotic therapy.
By providing reliable data on procalcitonin levels, this ELISA kit can aid in the treatment and management of infectious diseases in rats.Overall, the Rat PCT (Procalcitonin) ELISA Kit is a valuable tool for researchers studying inflammatory conditions in rats, offering a reliable method for quantifying procalcitonin levels and advancing our understanding of infectious diseases.
Product Name:
Rat PCT (Procalcitonin) ELISA Kit
SKU:
RTES01028
Target:
Rat PCT (Procalcitonin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PCT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PCT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PCT. You can calculate the concentration of Rat PCT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
94-107
85-96
Average (%)
96
102
91
1:4
Range (%)
101-113
87-98
96-113
Average (%)
107
92
103
1:8
Range (%)
97-114
84-94
95-111
Average (%)
104
89
102
1:16
Range (%)
100-112
81-94
99-114
Average (%)
106
86
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
102
EDTA plasma (n=5)
96-108
101
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.7
151.9
337.0
56.1
159.0
363.8
Standard deviation
3.1
8.2
17.2
3.6
7.2
17.1
C V (%)
6
5.4
5.1
6.42
4.53
4.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PCT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PCT in samples. No significant cross-reactivity or interference between Rat PCT and analogues was observed.
