The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a type? transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.
Product Name:
Rat IL4RA/CD124 Recombinant Protein (RPES4969)
Product Code:
RPES4969
Size:
100µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
IL4R,Il4ra
Accession:
Q63257-1
Sequence:
Met1-Arg232
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA.Immobilized mouse IL4-His at 10 µg/mL (100 ?l/well) can bind rat IL4R-Fc, The EC50 of rat IL4R-Fc is 0.12-0.29 µg/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat IL4R (Q63257-1) (Met1-Arg232) was expressed with the Fc region of human IgG1 at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
50.9 kDa
AP Mol Mass:
66 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
