The Rat Follicle-Stimulating Hormone (FSH) ELISA Kit is a cutting-edge tool for accurately measuring FSH levels in rat serum, plasma, and tissue culture supernatants. This kit is renowned for its superior sensitivity and specificity, guaranteeing dependable and consistent results, perfect for a diverse array of research endeavors.FSH is a pivotal hormone that plays a critical role in reproductive function, stimulating follicle development in the ovaries and regulating sperm production in males.
Dysregulation of FSH levels can lead to infertility, polycystic ovary syndrome, and other reproductive disorders, highlighting the significance of monitoring FSH levels.By utilizing the Rat FSH ELISA Kit, researchers can gain valuable insights into reproductive health, uncovering potential diagnostic markers and therapeutic targets for various reproductive conditions. Trust in this advanced kit to propel your research forward and unlock new discoveries in the field of reproductive biology.
Product Name:
Rat Follicle Stimulating Hormone (FSH) ELISA Kit
Product Code:
RTDL00396
Size:
96 Assays
Target:
FSH
Detection Method:
Competition
Range:
0.468-30mIU/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
