The Rat DBP (Vitamin D Binding Protein) ELISA Kit is a specialized assay designed for the accurate measurement of DBP levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.DBP, also known as vitamin D-binding protein, plays a crucial role in the transportation and regulation of vitamin D in the body. Studies have shown that DBP levels can be indicative of various disease states and conditions, making it a valuable biomarker for research in areas such as bone health, immune function, and inflammation.
This ELISA kit provides researchers with a reliable tool to study the role of DBP in various physiological and pathological processes, offering insights into potential therapeutic targets and interventions. With its high performance and versatility, the Rat DBP ELISA Kit is an essential tool for advancing research in the field of vitamin D biology and related areas.
Product Name:
Rat DBP (Vitamin D Binding Protein) ELISA Kit
SKU:
RTES00887
Target:
Rat DBP (Vitamin D Binding Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat DBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat DBP. You can calculate the concentration of Rat DBP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
89-101
90-103
Average (%)
99
95
97
1:4
Range (%)
89-104
81-93
88-102
Average (%)
95
88
94
1:8
Range (%)
90-102
83-93
84-95
Average (%)
97
88
89
1:16
Range (%)
86-101
81-90
85-95
Average (%)
92
85
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
93-110
100
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.41
85.83
484.15
48.96
87.95
440.22
Standard deviation
3.16
4.25
14.81
2.86
4.78
14.4
C V (%)
6.27
4.95
3.06
5.84
5.43
3.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat DBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DBP in samples. No significant cross-reactivity or interference between Rat DBP and analogues was observed.
