Rat Argininosuccinate Synthetase 1 (ASS1) ELISA Kit
The Rat Argininosuccinate Synthetase 1 (ASS1) ELISA Kit is specifically designed for the accurate and precise measurement of ASS1 levels in rat samples including serum, plasma, and tissue homogenates. This kit boasts high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.ASS1 is an important enzyme involved in the urea cycle and is critical for the production of arginine, a key amino acid for protein synthesis and various metabolic processes.
Dysregulation of ASS1 has been implicated in various diseases including cancer, liver disorders, and urea cycle disorders, making it a valuable biomarker for research and potential therapeutic developments.With the Rat Argininosuccinate Synthetase 1 (ASS1) ELISA Kit, researchers can accurately quantify ASS1 levels in rat samples, enabling a deeper understanding of its role in disease pathogenesis and providing valuable insights for potential treatment strategies.
Product Name:
Rat Argininosuccinate Synthetase 1 (ASS1) ELISA Kit
Product Code:
RTDL00115
Size:
96 Assays
Target:
ASS1
Synonyms:
CTLN1, Citrulline--aspartate ligase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
