Rat A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) ELISA Kit
The Rat ADAMTS1 (A Disintegrin and Metalloproteinase with Thrombospondin-1) ELISA Kit is specifically designed for the precise measurement of ADAMTS1 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for various research purposes.ADAMTS1 is a key enzyme involved in extracellular matrix remodeling and plays a crucial role in processes such as tissue development, inflammation, and wound healing.
Dysregulation of ADAMTS1 has been linked to various diseases, including arthritis, cancer, and cardiovascular disorders, highlighting its significance as a biomarker for studying these conditions and exploring potential therapeutic interventions.With its advanced technology and reliable performance, the Rat ADAMTS1 ELISA Kit is an invaluable tool for researchers seeking to deepen their understanding of ADAMTS1 biology and its implications in disease pathology.
Product Name:
Rat A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) ELISA Kit
Product Code:
RTDL00021
Size:
96 Assays
Target:
ADAMTS1
Synonyms:
C3-C5, METH1
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
