PSMD6 Antibody (PACO62043)
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주문- SKU:
- PACO62043
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | PSMD6 Antibody (PACO62043) |
Antibody SKU: | PACO62043 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Peptide sequence from Human 26S proteasome non-ATPase regulatory subunit 6 protein (355-372AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate. All lanes: PSMD6 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 42, 52 kDa. Observed band size: 46 kDa. |
![]() | Immunofluorescence staining of MCF-7 cells with PACO62043 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO62043 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. |
Synonyms: | 26S proteasome non-ATPase regulatory subunit 6 (26S proteasome regulatory subunit RPN7) (26S proteasome regulatory subunit S10) (Breast cancer-associated protein SGA-113M) (Phosphonoformate immuno-associated protein 4) (Proteasome regulatory particle subunit p44S10) (p42A), PSMD6, KIAA0107 PFAAP4 |
UniProt Protein Function: | PSMD6: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S10 family. |
UniProt Protein Details: | Chromosomal Location of Human Ortholog: 3p14.1 Cellular Component: nucleoplasm; proteasome complex; proteasome regulatory particle; cytosol Molecular Function:ATPase activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; proteolysis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle |
NCBI Summary: | This gene encodes a member of the protease subunit S10 family. The encoded protein is a subunit of the 26S proteasome which colocalizes with DNA damage foci and is involved in the ATP-dependent degradation of ubiquinated proteins. Alternative splicing results in multiple transcript variants [provided by RefSeq, Nov 2012] |
UniProt Code: | Q15008 |
NCBI GenInfo Identifier: | 7661914 |
NCBI Gene ID: | 9861 |
NCBI Accession: | NP_055629.1 |
UniProt Secondary Accession: | Q15008,Q6UV22, A8K2E0, E9PHI9, |
UniProt Related Accession: | Q15008 |
Molecular Weight: | 51,923 Da |
NCBI Full Name: | 26S proteasome non-ATPase regulatory subunit 6 isoform 2 |
NCBI Synonym Full Names: | proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 |
NCBI Official Symbol: | PSMD6 |
NCBI Official Synonym Symbols: | S10; Rpn7; p42A; p44S10; SGA-113M |
NCBI Protein Information: | 26S proteasome non-ATPase regulatory subunit 6; breast cancer-associated protein SGA-113M; phosphonoformate immuno-associated protein 4; proteasome regulatory particle subunit p44S10 |
UniProt Protein Name: | 26S proteasome non-ATPase regulatory subunit 6 |
UniProt Synonym Protein Names: | 26S proteasome regulatory subunit RPN7; 26S proteasome regulatory subunit S10; Breast cancer-associated protein SGA-113M; Phosphonoformate immuno-associated protein 4; Proteasome regulatory particle subunit p44S10; p42A |
UniProt Gene Name: | PSMD6 |
UniProt Entry Name: | PSMD6_HUMAN |