PREP Antibody (PACO62711)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO62711
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PREP Antibody (PACO62711) |
Antibody SKU: | PACO62711 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Prolyl endopeptidase protein (539-691AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: HL60 whole cell lysate. All lanes: PREP antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81 kDa. Observed band size: 81 kDa. |
![]() | Immunofluorescence staining of A549 cells with PACO62711 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO62711 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acid, long. |
Synonyms: | Prolyl endopeptidase (PE) (EC 3.4.21.26) (Post-proline cleaving enzyme), PREP, PEP |
UniProt Protein Function: | PREP: Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Belongs to the peptidase S9A family. |
UniProt Protein Details: | Protein type:EC 3.4.21.26; Protease Chromosomal Location of Human Ortholog: 6q22 Cellular Component: cytoplasm; membrane Molecular Function:protein binding; serine-type peptidase activity |
UniProt Code: | P48147 |
NCBI GenInfo Identifier: | 41349456 |
NCBI Gene ID: | 5550 |
NCBI Accession: | NP_002717.3 |
UniProt Secondary Accession: | P48147,Q8N6D4, |
UniProt Related Accession: | P48147 |
Molecular Weight: | 80,700 Da |
NCBI Full Name: | prolyl endopeptidase |
NCBI Synonym Full Names: | prolyl endopeptidase |
NCBI Official Symbol: | PREP |
NCBI Official Synonym Symbols: | PE; PEP |
NCBI Protein Information: | prolyl endopeptidase |
UniProt Protein Name: | Prolyl endopeptidase |
UniProt Synonym Protein Names: | Post-proline cleaving enzyme |
Protein Family: | Preprocaerulein |
UniProt Gene Name: | PREP |
UniProt Entry Name: | PPCE_HUMAN |
Antibodies | ELISA Kits |
Anti-PREP Antibody (CAB9838) | Human PREP (Prolyl endopeptidase) ELISA Kit (HUFI07064) |
PREP Antibody (PACO11457) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |