PPIE Antibody (PACO58592)
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주문- SKU:
- PACO58592
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PPIE Antibody (PACO58592) |
Antibody SKU: | PACO58592 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Peptidyl-prolyl cis-trans isomerase E protein (86-141AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO58592 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO58592 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing. |
Synonyms: | Peptidyl-prolyl cis-trans isomerase E (PPIase E) (EC 5.2.1.8) (Cyclophilin E) (Cyclophilin-33) (Rotamase E), PPIE, CYP33 |
UniProt Protein Function: | PPIE: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing. Belongs to the cyclophilin-type PPIase family. PPIase E subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Spliceosome; Cyclophilin; RNA splicing; EC 5.2.1.8; Isomerase Chromosomal Location of Human Ortholog: 1p32 Cellular Component: nucleoplasm; nucleus Molecular Function:cyclosporin A binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; RNA binding Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of viral genome replication; regulation of transcription, DNA-dependent; transcription-coupled nucleotide-excision repair |
NCBI Summary: | The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified. [provided by RefSeq, Aug 2010] |
UniProt Code: | Q9UNP9 |
NCBI GenInfo Identifier: | 13124097 |
NCBI Gene ID: | 10450 |
NCBI Accession: | Q9UNP9.1 |
UniProt Secondary Accession: | Q9UNP9,O43634, O43635, Q32Q72, Q3S611, Q5TGA0, Q5TGA2 Q5TGA3, Q9UIZ5, B2R971, |
UniProt Related Accession: | Q9UNP9 |
Molecular Weight: | 34,991 Da |
NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase E |
NCBI Synonym Full Names: | peptidylprolyl isomerase E |
NCBI Official Symbol: | PPIE |
NCBI Official Synonym Symbols: | CYP33; CYP-33 |
NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase E |
UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase E |
UniProt Synonym Protein Names: | Cyclophilin E; Cyclophilin-33; Rotamase E |
UniProt Gene Name: | PPIE |
UniProt Entry Name: | PPIE_HUMAN |
Antibodies |
Anti-PPIE Antibody (CAB7061) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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