PNPO Antibody (PACO57556)
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주문- SKU:
- PACO57556
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PNPO Antibody (PACO57556) |
Antibody SKU: | PACO57556 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Pyridoxine-5'-phosphate oxidase protein (114-207AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO57556 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of Hela cells with PACO57556 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO57556 diluted at 1:600 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). |
Synonyms: | Pyridoxine-5'-phosphate oxidase (EC 1.4.3.5) (Pyridoxamine-phosphate oxidase), PNPO |
UniProt Protein Function: | PNPO: Catalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). Defects in PNPO are the cause of pyridoxine-5'-phosphate oxidase deficiency (PNPO deficiency); also known as PNPO-related neonatal epileptic encephalopathy. The main feature of neonatal epileptic encephalopathy is the onset within hours of birth of a severe seizure disorder that does not respond to anticonvulsant drugs and can be fatal. Seizures can cease with the administration of PLP, being resistant to treatment with pyridoxine. Belongs to the pyridoxamine 5'-phosphate oxidase family. |
UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.4.3.5; Cofactor and Vitamin Metabolism - vitamin B6 Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: cytoplasm; cytosol; nucleoplasm Molecular Function:pyridoxamine-phosphate oxidase activity Biological Process: positive regulation of defense response to virus by host; pyridoxal phosphate biosynthetic process; vitamin B6 metabolic process Disease: Pyridoxamine 5-prime-phosphate Oxidase Deficiency |
NCBI Summary: | The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008] |
UniProt Code: | Q9NVS9 |
NCBI GenInfo Identifier: | 37082126 |
NCBI Gene ID: | 55163 |
NCBI Accession: | Q9NVS9.1 |
UniProt Secondary Accession: | Q9NVS9,B4E0V0, B4E152, B4E1D7, D3DTT9, |
UniProt Related Accession: | Q9NVS9 |
Molecular Weight: | 25,200 Da |
NCBI Full Name: | Pyridoxine-5'-phosphate oxidase |
NCBI Synonym Full Names: | pyridoxamine 5'-phosphate oxidase |
NCBI Official Symbol: | PNPO |
NCBI Official Synonym Symbols: | PDXPO; HEL-S-302 |
NCBI Protein Information: | pyridoxine-5'-phosphate oxidase |
UniProt Protein Name: | Pyridoxine-5'-phosphate oxidase |
UniProt Synonym Protein Names: | Pyridoxamine-phosphate oxidase |
Protein Family: | Pyridoxine-5'-phosphate oxidase |
UniProt Gene Name: | PNPO |
UniProt Entry Name: | PNPO_HUMAN |
Antibodies |
Anti-PNPO Antibody (CAB18495) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |