PLK3 Antibody (PACO22595)
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주문- SKU:
- PACO22595
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | PLK3 Antibody (PACO22595) |
Antibody SKU: | PACO22595 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
Species Reactivity: | Human |
Immunogen: | Synthesized peptide derived from internal of human PLK3. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot analysis of extracts from HepG2 cells, using PLK3 antibody. |
Background: | Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1. |
Synonyms: | EC 2.7.11.21; Polo-like kinase 3; PLK-3; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase |
UniProt Protein Function: | PLK3: a kinase of the PLK family. Phosphorylated and activated following DNA damage or mitotic spindle disruption. Interacts with Chk2 and the interaction is enhanced upon DNA damage. Apparent substrates include Chk2 and p53. Plays an important role in regulating microtubule dynamics and centrosomal function. Deregulated expression of Plk3 results in cell cycle arrest and apoptosis. |
UniProt Protein Details: | Protein type:Kinase, protein; Nucleolus; EC 2.7.11.21; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: centrosome; cytoplasm; Golgi stack; nucleolus; nucleoplasm; nucleus Molecular Function:p53 binding; protein binding; protein serine/threonine kinase activity Biological Process: apoptosis; cytoplasmic microtubule organization and biogenesis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; endomitotic cell cycle; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; mitotic cell cycle checkpoint; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; protein kinase B signaling cascade; regulation of cell division; regulation of cytokinesis; response to DNA damage stimulus; response to osmotic stress; response to radiation; response to reactive oxygen species |
NCBI Summary: | The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015] |
UniProt Code: | Q9H4B4 |
NCBI GenInfo Identifier: | 51338822 |
NCBI Gene ID: | 1263 |
NCBI Accession: | Q9H4B4.2 |
UniProt Secondary Accession: | Q9H4B4,Q15767, Q5JR99, Q96CV1, |
UniProt Related Accession: | Q9H4B4 |
Molecular Weight: | 71,629 Da |
NCBI Full Name: | Serine/threonine-protein kinase PLK3 |
NCBI Synonym Full Names: | polo like kinase 3 |
NCBI Official Symbol: | PLK3 |
NCBI Official Synonym Symbols: | CNK; FNK; PRK; PLK-3 |
NCBI Protein Information: | serine/threonine-protein kinase PLK3 |
UniProt Protein Name: | Serine/threonine-protein kinase PLK3 |
UniProt Synonym Protein Names: | Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3; Proliferation-related kinase |
Protein Family: | Serine/threonine-protein kinase |
UniProt Gene Name: | PLK3 |
UniProt Entry Name: | PLK3_HUMAN |