The PIVKA-II ELISA Kit is specifically designed for the precise and reliable detection of PIVKA-II levels in human serum samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for researchers studying conditions such as liver disease, vitamin K deficiency, and hepatocellular carcinoma.PIVKA-II, also known as protein induced by vitamin K absence or antagonist-II, is a protein produced in the liver that is elevated in individuals with impaired vitamin K metabolism.
Monitoring PIVKA-II levels can provide valuable insights into liver function and vitamin K status, making it a valuable biomarker for diagnostic and research purposes.With easy-to-follow instructions and all necessary components included, the PIVKA-II ELISA Kit is a convenient and efficient tool for detecting and quantifying PIVKA-II levels in human samples. Researchers can confidently rely on this kit for accurate and consistent results in their studies.
Product Name:
PIVKA-II ELISA Kit
SKU:
HUES03441
Target:
PIVKA-II
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIVKA-Ⅱ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PIVKA-Ⅱ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIVKA-Ⅱ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PIVKA-Ⅱ. You can calculate the concentration of Human PIVKA-Ⅱ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
94-107
98-111
Average (%)
94
99
105
1:4
Range (%)
86-100
83-98
87-103
Average (%)
93
90
94
1:8
Range (%)
91-103
79-92
82-95
Average (%)
97
86
89
1:16
Range (%)
89-100
82-98
83-98
Average (%)
94
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
92-103
97
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.03
4.96
0.44
1.01
4.78
Standard deviation
0.03
0.05
0.24
0.03
0.05
0.23
C V (%)
6.52
4.85
4.84
6.82
4.95
4.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PIVKA-Ⅱ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PIVKA-Ⅱ in samples. No significant cross-reactivity or interference between Human PIVKA-Ⅱ and analogues was observed.
