NF2 Antibody (PACO47570)
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주문- SKU:
- PACO47570
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | NF2 Antibody (PACO47570) |
Antibody SKU: | PACO47570 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Merlin protein (230-414AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: HEK293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: NF2 antibody at 2.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70, 73, 60, 65, 64, 31, 66, 20, 25 kDa. Observed band size: 70, 73 kDa.. | |
Immunofluorescent analysis of Hela cells using PACO47570 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human brain tissue using PACO47570 at dilution of 1:100. |
Background: | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-DCAF1/DCAF1 E3 ubiquitin-protein ligase complex. |
Synonyms: | Merlin (Moesin-ezrin-radixin-like protein) (Neurofibromin-2) (Schwannomerlin) (Schwannomin), NF2, SCH |
UniProt Protein Function: | Merlin: a moesin-ezrin-radizin-like protein. Interacts with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. Expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations are associated with neurofibromatosis type II. Two predominant isoforms and eight minor isoforms are produced by alternatively spliced transcripts. |
UniProt Protein Details: | Protein type:Tumor suppressor; Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: adherens junction; apical part of cell; cleavage furrow; cortical actin cytoskeleton; cytoplasm; cytoskeleton; early endosome; extrinsic to membrane; filopodium membrane; lamellipodium; lipid raft; membrane; neuron projection; nucleolus; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; synapse Molecular Function:actin binding; beta-catenin binding; integrin binding; protein binding; protein domain specific binding Biological Process: actin cytoskeleton organization and biogenesis; ectoderm development; hippocampus development; intercellular junction assembly and maintenance; mesoderm formation; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of cell-cell adhesion; negative regulation of cell-matrix adhesion; negative regulation of DNA replication; negative regulation of JAK-STAT cascade; negative regulation of MAPKKK cascade; negative regulation of protein kinase activity; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of tyrosine phosphorylation of Stat5 protein; odontogenesis of dentine-containing teeth; positive regulation of cell differentiation; positive regulation of stress fiber formation; regulation of gliogenesis; regulation of protein stability; Schwann cell proliferation; small GTPase mediated signal transduction Disease: Meningioma, Familial, Susceptibility To; Neurofibromatosis, Type Ii; Schwannomatosis 1 |
NCBI Summary: | This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008] |
UniProt Code: | P35240 |
NCBI GenInfo Identifier: | 462594 |
NCBI Gene ID: | 4771 |
NCBI Accession: | P35240.1 |
UniProt Secondary Accession: | P35240,O95683, Q8WUJ2, Q969N0, Q969Q3, Q96T30, Q96T31 Q96T32, Q96T33, Q9BTW3, Q9UNG9, Q9UNH3, |
UniProt Related Accession: | P35240 |
Molecular Weight: | 24,515 Da |
NCBI Full Name: | Merlin |
NCBI Synonym Full Names: | neurofibromin 2 (merlin) |
NCBI Official Symbol: | NF2 |
NCBI Official Synonym Symbols: | ACN; SCH; BANF |
NCBI Protein Information: | merlin |
UniProt Protein Name: | Merlin |
UniProt Synonym Protein Names: | Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin |
Protein Family: | Merlin |
UniProt Gene Name: | NF2 |
UniProt Entry Name: | MERL_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |