NECTIN4 Antibody (PACO59237)
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주문- SKU:
- PACO59237
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | NECTIN4 Antibody (PACO59237) |
Antibody SKU: | PACO59237 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Nectin-4 protein (91-199AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Raji whole cell lysate, NIH/3T3 whole cell lysate. All lanes: NECTIN4 antibody at 5.9µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56, 25 kDa. Observed band size: 35 kDa. |
![]() | Immunofluorescence staining of MCF-7 cells with PACO59237 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO59237 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells. |
Synonyms: | Nectin-4 (Ig superfamily receptor LNIR) (Nectin cell adhesion molecule 4) (Poliovirus receptor-related protein 4) [Cleaved into: Processed poliovirus receptor-related protein 4], NECTIN4, LNIR PRR4 PVRL4 |
UniProt Protein Function: | Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus. |
NCBI Summary: | This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011] |
UniProt Code: | Q96NY8 |
NCBI GenInfo Identifier: | 74761016 |
NCBI Gene ID: | 81607 |
NCBI Accession: | Q96NY8.1 |
UniProt Secondary Accession: | Q96NY8,Q96K15, B4DQW3, |
UniProt Related Accession: | Q96NY8 |
Molecular Weight: | 24,341 Da |
NCBI Full Name: | Nectin-4 |
NCBI Synonym Full Names: | nectin cell adhesion molecule 4 |
NCBI Official Symbol: | NECTIN4 |
NCBI Official Synonym Symbols: | LNIR; PRR4; EDSS1; PVRL4; nectin-4 |
NCBI Protein Information: | nectin-4 |
UniProt Protein Name: | Nectin-4 |
UniProt Synonym Protein Names: | Ig superfamily receptor LNIR; Nectin cell adhesion molecule 4 |
UniProt Gene Name: | NECTIN4 |
UniProt Entry Name: | NECT4_HUMAN |