The Mouse TGFB2 (Transforming Growth Factor Beta 2) ELISA Kit is specifically designed for the accurate quantification of TGFB2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for researchers in the field.TGFB2 is a key protein involved in regulating cell growth, differentiation, and immune responses. It plays a critical role in various biological processes, including tissue development, wound healing, and immune regulation.
Understanding TGFB2 levels can provide valuable insights into diseases such as cancer, fibrosis, and autoimmune disorders, making this ELISA kit a powerful tool for studying these conditions and developing potential therapies.Overall, the Mouse TGFB2 ELISA Kit from Assay Genie provides a reliable and efficient solution for researchers looking to investigate the role of TGFB2 in different physiological and pathological processes in mice.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TGF-β2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TGF-β2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TGF-β2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TGF-β2. You can calculate the concentration of Mouse TGF-β2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
89-104
85-97
Average (%)
103
97
90
1:4
Range (%)
91-102
84-97
84-100
Average (%)
96
89
91
1:8
Range (%)
93-108
80-94
85-99
Average (%)
99
86
92
1:16
Range (%)
85-100
86-98
85-96
Average (%)
92
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.37
84.5
499.92
49.73
76.67
544.03
Standard deviation
2.76
4.53
21.85
2.63
3.98
20.13
C V (%)
5.37
5.36
4.37
5.29
5.19
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TGF-β2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TGF-β2 in samples. No significant cross-reactivity or interference between Mouse TGF-β2 and analogues was observed.
