The Mouse TE (Telomerase) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of telomerase levels in mouse serum, plasma, and cell culture supernatants. Telomerase is an important enzyme that plays a key role in maintaining telomere length, which is crucial for cell proliferation and aging processes.This kit ensures high specificity and reproducibility, providing researchers with reliable results for a variety of research applications related to telomere biology and aging.
Telomerase dysfunction has been linked to various age-related diseases and cancers, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its user-friendly format and rapid assay procedure, the Mouse TE Telomerase ELISA Kit is a valuable tool for researchers looking to study telomerase activity and its implications in aging and disease processes in mouse models.
Product Name:
Mouse TE (telomerase) ELISA Kit
SKU:
MOES01533
Target:
Mouse TE (telomerase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TE. You can calculate the concentration of Mouse TE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
97-113
87-99
Average (%)
105
105
94
1:4
Range (%)
92-105
82-93
83-95
Average (%)
97
87
88
1:8
Range (%)
93-108
82-92
86-97
Average (%)
100
87
92
1:16
Range (%)
85-100
85-96
87-102
Average (%)
92
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
95
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.19
53.92
199.88
20.94
53.84
196.06
Standard deviation
1.04
2.3
8.1
1.24
2.41
10.18
C V (%)
5.15
4.27
4.05
5.92
4.48
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TE in samples. No significant cross-reactivity or interference between Mouse TE and analogues was observed.
