Mouse SH3YL1 (SH3 domain-containing YSC84-like protein 1) ELISA Kit
The Mouse SH3YL1 (SH3 Domain Containing YSC84 Like Protein 1) ELISA Kit is specifically designed for the accurate detection of SH3YL1 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for your research needs.SH3YL1 is a protein that plays a role in various cellular processes, including endocytosis and autophagy. It has been linked to certain diseases and conditions, making it a valuable biomarker for studying these pathways and developing potential treatments.
With the Mouse SH3YL1 ELISA Kit, researchers can confidently measure SH3YL1 levels in mouse samples, providing valuable insight into its function and potential implications in disease pathology. This kit is a valuable tool for advancing research in the field of cell biology and molecular medicine.
Product Name:
Mouse SH3YL1 (SH3 domain-containing YSC84-like protein 1) ELISA Kit
SKU:
MOES01825
Target:
Mouse SH3YL1 (SH3 domain-containing YSC84-like protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SH3YL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SH3YL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SH3YL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SH3YL1. You can calculate the concentration of Mouse SH3YL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
95-111
87-98
Average (%)
104
102
92
1:4
Range (%)
90-104
86-96
88-98
Average (%)
97
91
93
1:8
Range (%)
90-106
80-92
82-93
Average (%)
97
86
87
1:16
Range (%)
88-104
80-93
86-101
Average (%)
95
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.19
4.62
14.23
2.41
4.5
13.93
Standard deviation
0.15
0.23
0.61
0.16
0.24
0.66
C V (%)
6.85
4.98
4.29
6.64
5.33
4.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SH3YL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SH3YL1 in samples. No significant cross-reactivity or interference between Mouse SH3YL1 and analogues was observed.
