The Mouse SESN2 (Sestrin-2) CLIA Kit is specifically designed for the sensitive detection of Sestrin-2 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers high accuracy and precision, ensuring reliable and consistent results for a variety of research purposes.Sestrin-2 is a key protein involved in regulating cell metabolism and promoting cellular stress resistance. Its implications in conditions like cancer, cardiovascular diseases, and metabolic disorders make it a valuable biomarker for studying these diseases and exploring potential treatments.
Overall, the Mouse SESN2 CLIA Kit from AssayGenie provides researchers with a powerful tool to investigate the role of Sestrin-2 in various biological processes and disease states, offering valuable insights for future therapeutic advancements.
Product Name:
Mouse SESN2 (Sestrin 2) CLIA Kit
SKU:
MOES00529
Target:
Mouse SESN2 (Sestrin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SESN2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SESN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SESN2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SESN2. You can calculate the concentration of Mouse SESN2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
87-102
92-108
Average (%)
109
94
98
1:4
Range (%)
87-99
100-117
101-118
Average (%)
92
109
109
1:8
Range (%)
104-116
97-111
90-101
Average (%)
110
105
96
1:16
Range (%)
99-111
94-108
102-118
Average (%)
105
100
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
90-107
97
Cell culture media (n=5)
96-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.22
303.64
842.39
120.21
291.38
793.1
Standard deviation
13.34
28.45
61.49
12.97
21.5
89.38
C V (%)
12.1
9.37
7.3
10.79
7.38
11.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SESN2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SESN2 in samples. No significant cross-reactivity or interference between Mouse SESN2 and analogues was observed.
