Mouse PIAS1 (Protein Inhibitor Of Activated STAT 1) CLIA Kit
The Mouse PIAS1 (Protein Inhibitor of Activated STAT 1) CLIA Kit is a powerful tool for researchers looking to quantify levels of PIAS1 in mouse samples. PIAS1 is a key regulator of the STAT signaling pathway, modulating gene expression and modulating immune responses. This kit offers high sensitivity and specificity, allowing for precise measurements of PIAS1 levels in serum, plasma, and cell lysates. Its simple and efficient protocol makes it suitable for a wide range of experimental settings, providing reliable and reproducible results.
By accurately measuring PIAS1 levels, researchers can gain valuable insights into the mechanisms underlying inflammatory responses, immune regulation, and various disease conditions. This kit is essential for studying the role of PIAS1 in disease pathogenesis and for developing targeted therapies for STAT-related disorders.
Product Name:
Mouse PIAS1 (Protein Inhibitor Of Activated STAT 1) CLIA Kit
SKU:
MOES00508
Target:
Mouse PIAS1 (Protein Inhibitor Of Activated STAT 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PIAS1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PIAS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PIAS1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PIAS1. You can calculate the concentration of Mouse PIAS1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
99-112
99-115
Average (%)
97
107
105
1:4
Range (%)
88-101
98-111
92-103
Average (%)
95
104
98
1:8
Range (%)
101-115
100-115
102-118
Average (%)
107
108
108
1:16
Range (%)
98-110
84-97
90-105
Average (%)
104
90
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.21
230.82
867.88
98.04
241.21
842.6
Standard deviation
8.33
21.65
84.1
10.7
28.8
74.65
C V (%)
8.07
9.38
9.69
10.91
11.94
8.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PIAS1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PIAS1 in samples. No significant cross-reactivity or interference between Mouse PIAS1 and analogues was observed.
