The Mouse PCT (Procalcitonin) ELISA Kit is a valuable tool for researchers looking to accurately measure procalcitonin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.Procalcitonin is a marker of bacterial infection and sepsis, making it a critical biomarker for studying infectious diseases and inflammatory conditions in mice.
By accurately measuring procalcitonin levels, researchers can monitor disease progression, evaluate treatment efficacy, and develop new therapeutic strategies.Overall, the Mouse PCT ELISA Kit offers a convenient and efficient way to study procalcitonin levels in mouse models, advancing our understanding of infectious and inflammatory diseases.
Product Name:
Mouse PCT (Procalcitonin) ELISA Kit
SKU:
MOES01699
Target:
Mouse PCT (Procalcitonin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PCT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PCT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PCT. You can calculate the concentration of Mouse PCT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
86-98
89-99
Average (%)
96
93
94
1:4
Range (%)
102-114
85-96
93-107
Average (%)
108
90
100
1:8
Range (%)
98-114
83-96
93-106
Average (%)
105
88
98
1:16
Range (%)
96-111
81-94
94-107
Average (%)
104
87
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.6
111.2
489.1
55.1
102.2
447.8
Standard deviation
3.2
6.0
23.0
3.4
4.7
17.5
C V (%)
6.32
5.4
4.7
6.17
4.6
3.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PCT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PCT in samples. No significant cross-reactivity or interference between Mouse PCT and analogues was observed.
