The Mouse NRG-4 (Neuregulin-4) ELISA Kit is specifically designed for the precise measurement of Neuregulin-4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Neuregulin-4 is a key protein involved in various biological processes, including cell communication and development. It plays a crucial role in conditions such as cardiovascular diseases, metabolic disorders, and cancer, making it a valuable biomarker for investigating these conditions and exploring potential treatment options.
Overall, the Mouse NRG-4 ELISA Kit provides researchers with a reliable tool to study the role of Neuregulin-4 in different physiological and pathological processes, ultimately contributing to advancements in the field of biomedical research.
Product Name:
Mouse NRG-4 (Neuregulin 4) ELISA Kit
SKU:
MOES00713
Target:
Mouse NRG-4 (Neuregulin 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NRG-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NRG-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NRG-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NRG-4. You can calculate the concentration of Mouse NRG-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
85-99
89-103
Average (%)
100
91
95
1:4
Range (%)
93-106
85-100
88-103
Average (%)
98
91
95
1:8
Range (%)
87-101
86-97
83-99
Average (%)
93
92
90
1:16
Range (%)
91-103
81-92
83-94
Average (%)
97
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
92
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
2.98
8.33
1.15
2.69
7.96
Standard deviation
0.06
0.14
0.31
0.08
0.14
0.29
C V (%)
5.56
4.7
3.72
6.96
5.2
3.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NRG-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NRG-4 in samples. No significant cross-reactivity or interference between Mouse NRG-4 and analogues was observed.
