The Mouse NOS2 (iNOS, Nitric Oxide Synthase 2, Inducible) CLIA Kit is a reliable and accurate tool for detecting levels of NOS2 in mouse samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.NOS2 is an important enzyme that plays a key role in the production of nitric oxide, a signaling molecule involved in various physiological and pathological processes. Dysregulation of NOS2 is associated with inflammatory diseases, cancer, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.
With the Mouse NOS2 CLIA Kit, researchers can confidently measure NOS2 levels in serum, plasma, and tissue samples, enabling in-depth investigations into the role of this enzyme in health and disease. Trust in this kit for accurate and reproducible data that will drive forward your research efforts.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NOS2/iNOS. You can calculate the concentration of Mouse NOS2/iNOS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
103-118
88-101
Average (%)
97
110
94
1:4
Range (%)
99-116
93-104
101-117
Average (%)
107
99
109
1:8
Range (%)
97-110
89-102
98-115
Average (%)
104
96
105
1:16
Range (%)
94-111
92-105
101-116
Average (%)
101
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.23
560.81
1523.99
192.81
556.72
1413.08
Standard deviation
19.3
40.88
134.11
21.4
48.82
153.46
C V (%)
9.05
7.29
8.8
11.1
8.77
10.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NOS2/iNOS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed.
