The Mouse IL-35 ELISA Kit is specifically designed for the precise measurement of IL-35 levels in mouse serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit offers accurate and reproducible results, making it a valuable tool for researchers in various fields.IL-35 is a key cytokine that plays a critical role in regulating immune responses and inflammation. It has been implicated in a variety of autoimmune diseases, cancer, and infectious diseases, making it an important target for research and therapy development.
This ELISA kit provides a simple and reliable method for quantifying IL-35 levels in mouse samples, allowing researchers to better understand the role of this cytokine in disease pathogenesis and potentially identify new therapeutic targets. Order your Mouse IL-35 ELISA Kit today and take your research to the next level.
Product Name:
Mouse IL-35 ELISA Kit
SKU:
MOES01228
Target:
Mouse IL-35
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-35. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-35 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-35. You can calculate the concentration of Mouse IL-35 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-113
86-101
85-99
Average (%)
103
93
91
1:4
Range (%)
91-103
85-98
85-99
Average (%)
97
91
91
1:8
Range (%)
93-105
82-92
86-99
Average (%)
99
87
92
1:16
Range (%)
89-106
87-98
89-101
Average (%)
96
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.65
130.66
406.03
48.01
122.66
385.72
Standard deviation
2.98
6.48
19.08
2.77
5.79
13.23
C V (%)
5.77
4.96
4.7
5.77
4.72
3.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-35 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-35 in samples. No significant cross-reactivity or interference between Mouse IL-35 and analogues was observed.
