The Mouse IgG2b (Immunoglobulin G2b) ELISA Kit is a highly reliable and sensitive tool designed for the accurate detection of IgG2b levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and precision, ensuring trustworthy and reproducible results suitable for a variety of research applications.Immunoglobulin G2b is a critical component of the immune system, playing a key role in antibody-mediated responses and immune regulation.
It is involved in various immune processes, including immune response modulation and inflammation regulation, making it a valuable biomarker for studying immunological conditions and developing potential therapies.Overall, the Mouse IgG2b (Immunoglobulin G2b) ELISA Kit is an essential tool for researchers looking to accurately measure IgG2b levels in mouse samples and further understand the immune system's functions and responses.
Product Name:
Mouse IgG2b (Immunoglobulin G2b) ELISA Kit
SKU:
MOES01680
Target:
Mouse IgG2b (Immunoglobulin G2b)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG2b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG2b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG2b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG2b. You can calculate the concentration of Mouse IgG2b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
93-106
87-102
Average (%)
98
99
93
1:4
Range (%)
89-103
81-94
84-96
Average (%)
95
86
89
1:8
Range (%)
88-103
84-97
85-97
Average (%)
94
90
91
1:16
Range (%)
88-98
82-93
82-93
Average (%)
93
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.7
26.94
84.35
10.77
27.67
77.31
Standard deviation
0.59
1.36
2.67
0.74
1.28
2.67
C V (%)
5.51
5.05
3.17
6.87
4.63
3.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IgG2b concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgG2b in samples. No significant cross-reactivity or interference between Mouse IgG2b and analogues was observed.
