The Mouse GDF3 (Growth Differentiation Factor 3) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of GDF3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers accurate and reproducible results, making it ideal for a variety of research applications.GDF3 is an important protein that plays a role in growth and differentiation processes, making it a key molecule in developmental biology and regenerative medicine research.
Its dysregulation has been implicated in various diseases, highlighting the significance of studying GDF3 levels in different biological samples.With the Mouse GDF3 CLIA Kit, researchers can accurately measure GDF3 levels, gaining valuable insights into its functions and potential therapeutic implications. This kit is a valuable tool for enhancing our understanding of GDF3 biology and its impact on health and disease.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDF3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GDF3. You can calculate the concentration of Mouse GDF3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
88-98
94-108
Average (%)
99
93
101
1:4
Range (%)
89-100
94-108
90-104
Average (%)
94
102
97
1:8
Range (%)
100-117
101-113
98-114
Average (%)
107
106
105
1:16
Range (%)
94-107
86-99
94-112
Average (%)
99
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
104
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
96-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.12
119.75
468.33
53.84
125.67
447.52
Standard deviation
4.06
12.14
32.6
5.17
12.45
42.2
C V (%)
8.1
10.14
6.96
9.6
9.91
9.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GDF3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDF3 in samples. No significant cross-reactivity or interference between Mouse GDF3 and analogues was observed.
