The Mouse GDF11 (Growth Differentiation Factor 11) CLIA Kit is a valuable tool for researchers looking to accurately assess GDF11 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GDF11 is a key regulator of growth and differentiation in various tissues, with important roles in muscle regeneration, aging, and cardiovascular health.
By detecting GDF11 levels, researchers can gain insights into its impact on different biological processes and potential implications for therapeutic development.Overall, the Mouse GDF11 CLIA Kit provides a reliable and efficient method for studying the function of GDF11 in mouse models, offering valuable information for furthering understanding of its biological significance and potential clinical applications.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GDF11. You can calculate the concentration of Mouse GDF11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
85-96
92-103
Average (%)
100
91
97
1:4
Range (%)
93-108
101-116
99-113
Average (%)
98
106
105
1:8
Range (%)
98-113
98-112
84-97
Average (%)
105
106
91
1:16
Range (%)
92-105
91-102
94-106
Average (%)
98
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-113
104
EDTA plasma (n=5)
96-112
103
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.99
78.64
169.85
24.98
81.66
164.83
Standard deviation
2.4
8.3
14.88
3.12
9.38
11.32
C V (%)
9.6
10.55
8.76
12.49
11.49
6.87
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GDF11 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDF11 in samples. No significant cross-reactivity or interference between Mouse GDF11 and analogues was observed.
