The Mouse FMOD (Fibromodulin) CLIA Kit is a highly sensitive and specific assay designed for the detection of fibromodulin levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an ideal tool for research applications.Fibromodulin is a key protein involved in extracellular matrix organization and regulation, playing a crucial role in tissue development and repair.
Dysregulation of fibromodulin has been implicated in various diseases such as arthritis, cardiovascular diseases, and fibrosis, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Mouse FMOD CLIA Kit offers researchers a reliable and precise method for measuring fibromodulin levels in mouse samples, contributing to a better understanding of its role in health and disease.
Product Name:
Mouse FMOD (Fibromodulin) CLIA Kit
SKU:
MOES00266
Target:
Mouse FMOD (Fibromodulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FMOD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FMOD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FMOD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FMOD. You can calculate the concentration of Mouse FMOD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-101
85-97
103-117
Average (%)
96
91
109
1:4
Range (%)
91-103
96-110
98-110
Average (%)
97
103
105
1:8
Range (%)
87-97
91-102
89-99
Average (%)
92
96
94
1:16
Range (%)
88-100
95-108
88-103
Average (%)
93
102
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.67
11.53
37.31
5.07
11.72
38.47
Standard deviation
0.38
1.05
3.98
0.56
1.18
2.63
C V (%)
8.14
9.11
10.67
11.05
10.07
6.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FMOD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FMOD in samples. No significant cross-reactivity or interference between Mouse FMOD and analogues was observed.
