The Mouse CD8a Monoclonal Antibody (PE-conjugated, 53-6.7) (AGEL0381) is a powerful tool for immunological research, specifically targeting the CD8a protein found on the surface of cytotoxic T cells. This antibody, derived from hybridoma clone 53-6.7, is conjugated with phycoerythrin (PE) for easy detection and fluorescence-based analyses. CD8a is a crucial marker for identifying cytotoxic T cells, a key component of the adaptive immune response responsible for targeting and eliminating infected or abnormal cells. By specifically binding to CD8a, this antibody allows for precise identification and characterization of cytotoxic T cells in various experimental setups, including flow cytometry and immunofluorescence assays.
Researchers studying immune responses, infectious diseases, cancer immunotherapy, and autoimmunity will find this monoclonal antibody invaluable for investigating the role of cytotoxic T cells in these contexts. Its high specificity and sensitivity make it a reliable tool for exploring immune cell populations and their functions, providing valuable insights into the complex mechanisms underlying immune system function.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P01731
Gene ID:
12525
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
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