The Mouse CD25 Monoclonal Antibody (AF647 Conjugated, PC61.5.3, AGEL1304) is a valuable tool for researchers studying immune responses and T cell regulation. This antibody, derived from mouse hybridoma cells, specifically targets the CD25 protein, also known as the interleukin-2 receptor alpha chain.CD25 is a crucial molecule involved in T cell activation and immune tolerance. Its expression is essential for the development and function of regulatory T cells, which play a key role in maintaining immune homeostasis and preventing autoimmunity. The AF647 conjugation enables efficient detection of CD25 in flow cytometry and immunofluorescence applications.By utilizing the Mouse CD25 Monoclonal Antibody, researchers can gain insights into the dynamics of T cell activation, immune suppression, and autoimmune diseases.
This antibody is an invaluable tool for investigating mechanisms of immune regulation and developing targeted therapies for conditions such as transplantation rejection, autoimmune disorders, and cancer immunotherapy.For robust and reliable results in T cell research, the Mouse CD25 Monoclonal Antibody (AF647 Conjugated, PC61.5.3, AGEL1304) offers high specificity and sensitivity, making it a preferred choice for immunologists and cell biologists exploring the intricate pathways of immune modulation.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.