PE/Cyanine7 Anti-Mouse CD162 Antibody [4RA10] (AGEL0843)

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SKU:
AGEL0843
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Applications:
FC
Frequently bought together:

Description

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PE/Cyanine7 Anti-Mouse CD162 Antibody [4RA10]

The Mouse CD162 Monoclonal Antibody (PE/Cyanine7 Conjugated, 4RA10) is a specialized tool for researchers studying CD162, a cell surface molecule known to play a role in immune responses and cell adhesion. This antibody, specifically designed for use with mouse samples, is conjugated with PE/Cyanine7, enabling multicolor flow cytometry applications for the detection and analysis of CD162 expression in various cell populations. Its high specificity and sensitivity make it an ideal choice for immunology research, particularly in the fields of inflammation, immune cell signaling, and cellular adhesion mechanisms.CD162, also referred to as PSGL-1 (P-selectin glycoprotein ligand-1), is recognized for its involvement in leukocyte trafficking and interaction with selectins on endothelial cells during inflammatory responses.

The precise targeting of this molecule with the Mouse CD162 Monoclonal Antibody allows for in-depth investigations into the mechanisms underlying immune cell adhesion and migration, which are essential processes in inflammation, infection, and immune system regulation. This antibody serves as a valuable tool for elucidating the functions of CD162 in various experimental settings, contributing to advancements in understanding immune cell behavior and potential therapeutic interventions in diseases characterized by dysregulated immune responses.

Product Title:PE/Cyanine7 Anti-Mouse CD162 Antibody [4RA10]
SKU:AGEL0843
Size:50 Tests, 100 Tests, 200 Tests
Clone No:4RA10
Synonyms:P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG1, κ
Isotype Control:PE/Cyanine7 Rat IgG1, κ Isotype Control[HRPN]
Form:Liquid
Conjugation:PE/Cyanine 7
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:Spectrum Image
Swissprot:Q62170
Gene ID:20345
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

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