The Mouse CD16/32 Monoclonal Antibody (PerCP-Cyanine5.5 Conjugated, 2.4G2) from AssayGenie (AGEL0594) is a highly specific antibody designed for research applications involving CD16 and CD32, also known as Fc receptors. These receptors play essential roles in mediating immune responses and are commonly found on various immune cells, such as macrophages, dendritic cells, and natural killer cells. This monoclonal antibody, conjugated with PerCP-Cyanine5.5, offers high sensitivity and specificity for detecting and analyzing CD16 and CD32 proteins in a range of cell types and experimental conditions.
By targeting these Fc receptors, researchers can investigate immune cell activation, antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, and other immune functions. This antibody is a valuable tool for studies in immunology, infectious diseases, and cancer research, providing insights into the mechanisms underlying immune responses and potential therapeutic targets. Its reliability and performance make it an essential reagent for scientists studying the intricate functions of Fc receptors in the immune system.
CD16a/b;CD32;CD32A/B;Fc fragment of IgG low affinity IIa/b receptor;Fc fragment of IgG low affinity IIIa/b receptor;Fc fragment of IgG low affinity IIIb receptor;Fc gamma receptor III A/B;FCG2A;FcGR;FCGR2A/BFCGR3;FCGR3A/B;Fc gamma RIIa/b ;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG2b, κ
Isotype Control:
PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
PerCP/Cyanine 5.5
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P08508 P08101
Gene ID:
14130,14131
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.
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