The Mouse Casp1 (Caspase-1) ELISA Kit is specifically designed for the quantitative detection of mouse caspase-1 levels in various samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reliable results for research purposes.Caspase-1 is an important enzyme involved in inflammation and immune responses, playing a key role in the activation of pro-inflammatory cytokines. Dysregulation of caspase-1 has been linked to various diseases, including autoimmune disorders and inflammatory conditions, making it a valuable biomarker for studying and understanding these pathologies.
This ELISA kit provides a valuable tool for researchers looking to investigate the role of caspase-1 in disease pathways and developing potential therapeutic interventions. Its easy-to-use format, along with its high performance, makes it an essential component in any research laboratory studying inflammation and immune-related conditions in mice.
Product Name:
Mouse CASP1 (Caspase 1) ELISA Kit
SKU:
MOES00780
Target:
Mouse CASP1 (Caspase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CASP1. You can calculate the concentration of Mouse CASP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
96-114
85-100
Average (%)
105
104
92
1:4
Range (%)
92-108
83-96
89-100
Average (%)
100
88
94
1:8
Range (%)
88-98
84-97
85-100
Average (%)
93
89
92
1:16
Range (%)
89-103
86-97
82-94
Average (%)
94
91
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.71
242.59
992.79
98.49
242.22
1084.14
Standard deviation
5.82
12.01
54.31
5.53
11.75
47.27
C V (%)
6.21
4.95
5.47
5.61
4.85
4.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CASP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CASP1 in samples. No significant cross-reactivity or interference between Mouse CASP1 and analogues was observed.
