MIP Antibody (PACO57860)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO57860
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | MIP Antibody (PACO57860) |
Antibody SKU: | PACO57860 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Lens fiber major intrinsic protein (220-263AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO57860 diluted at 1:200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of HepG2 cells with PACO57860 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO57860 diluted at 1:200 and staining in paraffin-embedded human eye tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Water channel. Channel activity is down-regulated by CALM when cytoplasmic Ca(2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core (By similarity). Plays a role in cell-to-cell adhesion and facilitates gap junction coupling. |
Synonyms: | Lens fiber major intrinsic protein (Aquaporin-0) (MIP26) (MP26), MIP, AQP0 |
UniProt Protein Function: | AQP0: major intrinsic protein of lens fiber. A member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. May be responsible for regulating the osmolarity of the lens. Defects in MIP are a cause of autosomal recessive congenital cataract. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.; Transporter, aquaporin family Chromosomal Location of Human Ortholog: 12q13 Cellular Component: intracellular canaliculus; intracellular membrane-bound organelle; integral to plasma membrane; apical plasma membrane; plasma membrane; gap junction Molecular Function:calmodulin binding; transporter activity; water channel activity; structural constituent of eye lens; glycerol channel activity Biological Process: lens development in camera-type eye; glycerol transport; cellular water homeostasis; visual perception; canalicular bile acid transport; water transport; response to stimulus; transmembrane transport Disease: Cataract 15, Multiple Types |
NCBI Summary: | Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13. [provided by RefSeq, Jul 2008] |
UniProt Code: | P30301 |
NCBI GenInfo Identifier: | 266537 |
NCBI Gene ID: | 4284 |
NCBI Accession: | P30301.1 |
UniProt Secondary Accession: | P30301,Q17R41, |
UniProt Related Accession: | P30301 |
Molecular Weight: | 263 |
NCBI Full Name: | Lens fiber major intrinsic protein |
NCBI Synonym Full Names: | major intrinsic protein of lens fiber |
NCBI Official Symbol: | MIP |
NCBI Official Synonym Symbols: | AQP0; LIM1; MP26; MIP26; CTRCT15 |
NCBI Protein Information: | lens fiber major intrinsic protein; aquaporin 0 |
UniProt Protein Name: | Lens fiber major intrinsic protein |
UniProt Synonym Protein Names: | Aquaporin-0; MIP26; MP26 |
Protein Family: | Mipartoxin |
UniProt Gene Name: | MIP |
UniProt Entry Name: | MIP_HUMAN |