ME1 Antibody (PACO22453)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO22453
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | ME1 Antibody (PACO22453) |
Antibody SKU: | PACO22453 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
Species Reactivity: | Human |
Immunogen: | Synthesized peptide derived from internal of human ME1. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from HeLa cells, using ME1 antibody. |
Background: | This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. |
Synonyms: | NADP-dependent malic enzyme; EC 1.1.1.40; NADP-ME; Malic enzyme 1; |
UniProt Protein Function: | ME1: a ubiquitous lipogenic enzyme of the malic enzymes family. Converts (S)-malate + NADP+ ?? pyruvate + CO2 + NADPH. Levels of ME1 and ATP-citrate lyase mRNA, another lipogenic enzyme, are coordinately increased 5-7 fold during the differentiation of 3T3-L1 preadipocytes into adipocytes. Up-regulated by short-term dietary restriction in mice. The cycling of pyruvate by ME1 generates cytosolic NADPH, whereas mitochondrial ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting. |
UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.1.1.40; Carbohydrate Metabolism - pyruvate Chromosomal Location of Human Ortholog: 6q12 Cellular Component: mitochondrion; cytosol Molecular Function:malate dehydrogenase (decarboxylating) activity; malic enzyme activity; electron carrier activity; manganese ion binding; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; ADP binding; NADP binding; NAD binding; oxaloacetate decarboxylase activity Biological Process: malate metabolic process; carbohydrate metabolic process; response to hormone stimulus; NADP biosynthetic process; cellular lipid metabolic process; response to carbohydrate stimulus; protein tetramerization |
NCBI Summary: | This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008] |
UniProt Code: | P48163 |
NCBI GenInfo Identifier: | 1346484 |
NCBI Gene ID: | 4199 |
NCBI Accession: | P48163.1 |
UniProt Secondary Accession: | P48163,Q16797, Q16855, Q53F72, Q5VWA2, Q9BWX8, Q9H1W3 Q9UIY4, B4DZ70, |
UniProt Related Accession: | P48163 |
Molecular Weight: | 572 |
NCBI Full Name: | NADP-dependent malic enzyme |
NCBI Synonym Full Names: | malic enzyme 1, NADP(+)-dependent, cytosolic |
NCBI Official Symbol: | ME1 |
NCBI Official Synonym Symbols: | MES; HUMNDME |
NCBI Protein Information: | NADP-dependent malic enzyme; NADP-ME; malate dehydrogenase; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; pyruvic-malic carboxylase |
UniProt Protein Name: | NADP-dependent malic enzyme |
UniProt Synonym Protein Names: | Malic enzyme 1 |
Protein Family: | NADP-dependent malic enzyme |
UniProt Gene Name: | ME1 |
UniProt Entry Name: | MAOX_HUMAN |