MAP4K4 Antibody (PACO23183)
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주문- SKU:
- PACO23183
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MAP4K4 Antibody (PACO23183) |
Antibody SKU: | PACO23183 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IF:1:100-1:500 |
Species Reactivity: | Human, Mouse |
Immunogen: | Synthesized peptide derived from internal of human MEKKK 4. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from COLO cells and HUVEC cells, using MEKKK 4 antibody. | |
Immunofluorescence analysis of A549 cells, using MEKKK 4 antibody. |
Background: | Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322. |
Synonyms: | EC 2.7.11.1; HPK/GCK-like kinase HGK; M4K4; MAP4K4; MAPK/ERK kinase kinase kinase 4 |
UniProt Protein Function: | HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Protein kinase, STE; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MSN subfamily Chromosomal Location of Human Ortholog: 2q11.2-q12 Cellular Component: cytoplasm Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: microvillus biogenesis; regulation of catalytic activity; protein amino acid phosphorylation; regulation of JNK cascade |
NCBI Summary: | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
UniProt Code: | O95819 |
NCBI GenInfo Identifier: | 29427585 |
NCBI Gene ID: | 9448 |
NCBI Accession: | O95819.2 |
UniProt Secondary Accession: | O95819,O75172, Q9NST7, |
UniProt Related Accession: | O95819 |
Molecular Weight: | 1239 |
NCBI Full Name: | Mitogen-activated protein kinase kinase kinase kinase 4 |
NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase kinase 4 |
NCBI Official Symbol: | MAP4K4 |
NCBI Official Synonym Symbols: | HGK; NIK; MEKKK4; FLH21957; HEL-S-31 |
NCBI Protein Information: | mitogen-activated protein kinase kinase kinase kinase 4; MEK kinase kinase 4; nck-interacting kinase; HPK/GCK-like kinase HGK; Ste20 group protein kinase HGK; MAPK/ERK kinase kinase kinase 4; epididymis secretory protein Li 31; hepatocyte progenitor kinase-like/germinal center kinase-like kinase |
UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase kinase 4 |
UniProt Synonym Protein Names: | HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase |
Protein Family: | Mitogen-activated protein kinase kinase kinase kinase |
UniProt Gene Name: | MAP4K4 |
UniProt Entry Name: | M4K4_HUMAN |