LIMK2 Antibody (PACO23746)
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주문- SKU:
- PACO23746
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | LIMK2 Antibody (PACO23746) |
Antibody SKU: | PACO23746 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Synthesized peptide derived from internal of human LIMK2. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot analysis of extracts from HepG2 cells and HUVEC cells, using LIMK2 antibody. |
![]() | Immunofluorescence analysis of HeLa cells, using LIMK2 antibody. |
![]() | Immunohistochemistry analysis of paraffin-embedded human heart tissue using LIMK2 antibody. |
Background: | Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. |
Synonyms: | EC 2.7.11.1; LIM domain kinase 2; LIMK-2; kinase LIMK2; |
UniProt Protein Function: | LIMK2: a TKL kinase of the LISK family that regulates actin dynamics. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. Phosphorylates cofilin, inhibiting its actin-depolymerizing activity. May contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants have been identified. |
UniProt Protein Details: | Protein type:Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; TKL group; LISK family; LIMK subfamily Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: cis-Golgi network; cytoplasm; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; ATP binding Biological Process: spermatogenesis; phosphorylation; protein amino acid phosphorylation |
NCBI Summary: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P53671 |
NCBI GenInfo Identifier: | 1708824 |
NCBI Gene ID: | 3985 |
NCBI Accession: | P53671.1 |
UniProt Secondary Accession: | P53671,Q7KZ80, Q7L3H5, Q96E10, Q99464, Q9UFU0, A8K6H5 |
UniProt Related Accession: | P53671 |
Molecular Weight: | 638 |
NCBI Full Name: | LIM domain kinase 2 |
NCBI Synonym Full Names: | LIM domain kinase 2 |
NCBI Official Symbol: | LIMK2 |
NCBI Protein Information: | LIM domain kinase 2 |
UniProt Protein Name: | LIM domain kinase 2 |
Protein Family: | LIM domain kinase |
UniProt Gene Name: | LIMK2 |
UniProt Entry Name: | LIMK2_HUMAN |
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