LGR4 Antibody (PACO56206)
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주문- SKU:
- PACO56206
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | LGR4 Antibody (PACO56206) |
Antibody SKU: | PACO56206 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 4 protein (234-365AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO56206 diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with PACO56206 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (By similarity). |
Synonyms: | Leucine-rich repeat-containing G-protein coupled receptor 4 (G-protein coupled receptor 48), LGR4, GPR48 |
UniProt Protein Function: | LGR4: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Plays an essential role during homeostasis of the adult mouse intestine. |
UniProt Protein Details: | Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 11p14.1 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:G-protein coupled receptor activity; protein binding; transmembrane receptor activity Biological Process: bone mineralization; bone remodeling; circadian regulation of gene expression; negative regulation of cytokine secretion; negative regulation of toll-like receptor signaling pathway; osteoblast differentiation; spermatogenesis Disease: Bone Mineral Density Quantitative Trait Locus 17 |
NCBI Summary: | The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body. [provided by RefSeq, Oct 2016] |
UniProt Code: | Q9BXB1 |
NCBI GenInfo Identifier: | 212286375 |
NCBI Gene ID: | 55366 |
NCBI Accession: | Q9BXB1.2 |
UniProt Secondary Accession: | Q9BXB1,Q8N537, Q9NYD1, A6NCH3, G5E9B3, |
UniProt Related Accession: | Q9BXB1 |
Molecular Weight: | 104kDa |
NCBI Full Name: | Leucine-rich repeat-containing G-protein coupled receptor 4 |
NCBI Synonym Full Names: | leucine rich repeat containing G protein-coupled receptor 4 |
NCBI Official Symbol: | LGR4 |
NCBI Official Synonym Symbols: | GPR48; BNMD17 |
NCBI Protein Information: | leucine-rich repeat-containing G-protein coupled receptor 4 |
UniProt Protein Name: | Leucine-rich repeat-containing G-protein coupled receptor 4 |
UniProt Synonym Protein Names: | G-protein coupled receptor 48 |
Protein Family: | Leucine-rich repeat-containing G-protein coupled receptor |
UniProt Gene Name: | LGR4 |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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