Human VASPIN (Visceral Adipose Specific Serine Protease Inhibitor) ELISA Kit
The Human Vaspin ELISA Kit is a valuable tool for detecting levels of visceral adipose-specific serine protease inhibitor in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.Vaspin, also known as visceral adipose-specific serine protease inhibitor, is a protein that plays a key role in regulating adipose tissue function and metabolism. Its dysregulation has been associated with obesity, insulin resistance, and other metabolic disorders, making it an important target for studying and developing therapies for these conditions.
By using the Human Vaspin ELISA Kit, researchers can gain valuable insights into the role of vaspin in metabolic health and disease, advancing our understanding of obesity-related complications and potential treatment strategies. Trust in the accuracy and precision of this kit for your research needs.
Product Name:
Human VASPIN (Visceral Adipose Specific Serine Protease Inhibitor) ELISA Kit
SKU:
HUES02740
Target:
Human VASPIN (Visceral Adipose Specific Serine Protease Inhibitor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VASPIN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VASPIN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VASPIN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VASPIN. You can calculate the concentration of Human VASPIN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
85-97
95-109
Average (%)
100
90
100
1:4
Range (%)
91-106
85-99
84-99
Average (%)
98
91
91
1:8
Range (%)
87-99
84-94
88-102
Average (%)
93
89
94
1:16
Range (%)
90-104
79-94
84-95
Average (%)
95
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-106
101
EDTA plasma (n=5)
92-107
99
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
195.45
361.96
1923.05
199.24
351.56
1798.47
Standard deviation
13.15
16.72
86.54
11.14
15.19
76.08
C V (%)
6.73
4.62
4.5
5.59
4.32
4.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VASPIN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VASPIN in samples. No significant cross-reactivity or interference between Human VASPIN and analogues was observed.
