Human UMPS Recombinant Protein (RPPB2405)
- SKU:
- RPPB2405
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- UMPS
- Synonyms:
- Uridine Monophosphate Synthetase
- UMP Synthase
- Orotate Phosphoribosyl Transferase And Orotidine-5-Decarboxylase
- Orotidine 5-Phosphate Decarboxylase
- Source:
- Sf9 Insect cells
- Uniprot:
- P11172
Description
| Product Name: | Human UMPS Recombinant Protein |
| Product Code: | RPPB2405 |
| Size: | 10µg |
| Species: | Human |
| Target: | UMPS |
| Synonyms: | Uridine Monophosphate Synthetase, UMP Synthase, Orotate Phosphoribosyl Transferase And Orotidine-5-Decarboxylase, Orotidine 5-Phosphate Decarboxylase, Orotate Phosphoribosyltransferase, Uridine 5-Monophosphate Synthase, OMPdecase, OPRTase, OPRT, Uridine 5'-monophosphate synthase, UMP synthase, Orotate phosphoribosyltransferase, Orotidine 5'-phosphate decarboxylase, ODC. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | UMPS protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4). |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAVARAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADILFQT AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL IIEDVVTSGS SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML EILEQQKKVD AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFGAR AELPRIHPVA SKLLRLMQKK ETNLCLSADV SLARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT LAKCHEFLIF EDRKFADIGN TVKKQYEGGI FKIASWADLV NAHVVPGSGV VKGLQEVGLP LHRGCLLIAE MSSTGSLATG DYTRAAVRMA EEHSEFVVGF ISGSRVSMKP EFLHLTPGVQ LEAGGDNLGQ QYNSPQEVIG KRGSDIIIVG RGIISAADRL EAAEMYRKAA WEAYLSRLGV HHHHHH |
Uridine 5'-monophosphate synthase (UMPS), is a bifunctional enzyme that catalyzes the ultimate two steps of the de novo pyrimidine biosynthetic pathway. UMPS in eukaryotes links the orotate phosphoribosyltransferase and the orotidine-5’-monophosphate (OMP) decarboxylase activities into a single protein. The harmony of these 2 enzymes is assumed to be stabilized the catalytic centers as a result of the low molar concentration of the protein in mammalian cells. Mutations in UMPS are the reason of inherited orotic aciduria disease.
UMPS Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 486 amino acids (1-480 a.a.) and having a molecular mass of 53kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). UMPS is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UMPS: Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1). A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.4.2.10; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Lyase; EC 4.1.1.23; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 3q13 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:orotate phosphoribosyltransferase activity; orotidine-5'-phosphate decarboxylase activity Biological Process: pyrimidine nucleoside biosynthetic process; UMP biosynthetic process Disease: Orotic Aciduria |
| NCBI Summary: | This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| UniProt Code: | P11172 |
| NCBI GenInfo Identifier: | 131708 |
| NCBI Gene ID: | 7372 |
| NCBI Accession: | P11172.1 |
| UniProt Secondary Accession: | P11172,O00758, O00759, O00760, Q16862, Q9H3Q2, Q9UG49 B5LY68, B5LY72, |
| UniProt Related Accession: | P11172 |
| Molecular Weight: | 22,922 Da |
| NCBI Full Name: | Uridine 5'-monophosphate synthase |
| NCBI Synonym Full Names: | uridine monophosphate synthetase |
| NCBI Official Symbol: | UMPS |
| NCBI Official Synonym Symbols: | OPRT |
| NCBI Protein Information: | uridine 5'-monophosphate synthase |
| UniProt Protein Name: | Uridine 5'-monophosphate synthase |
| UniProt Synonym Protein Names: | Orotate phosphoribosyltransferase (EC:2.4.2.10); OPRT; OPRTaseOrotidine 5'-phosphate decarboxylase (EC:4.1.1.23); ODCAlternative name(s):OMPdecase |
| Protein Family: | Uridine 5'-monophosphate synthase |
| UniProt Gene Name: | UMPS |
| UniProt Entry Name: | UMPS_HUMAN |
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